Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband®

About this product

Boster Bio Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband® catalog # A01065. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01065

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Methylmalonyl-CoA mutase (MUT) is a mitochondrial enzyme that catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA. This gene is mapped to 6p12.3. MUT requires a vitamin B12-derived prosthetic group, adenosylcobalamin (commonly referred to as AdoCbl), to function. And the product of this enzyme, succinyl-CoA, is a key molecule of the TCA cycle.

Synonyms

Methylmalonyl-CoA mutase, mitochondrial; MCM;5.4.99.2 ; Methylmalonyl-CoA isomerase; MUT

Gene Name

MUT

Gene ID

4594

UniProt

P22033

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human MUT, different from the related mouse sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

T-cell specific.

Applications

Flow Cytometry, IHC, WB

Field of Research

Apoptosis, Cancer, Cancer Susceptibility, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Chromatin Binding Proteins, DNA/RNA, DNA/RNA Binding, DNA Damage & Repair, DNA Damage Response, Epigenetics and Nuclear Signaling, Intracellular, Oncoproteins/Suppressors, p53 Pathway, Transcription, Tumor Suppressors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Involved in the degradation of several amino acids, odd- chain fatty acids and cholesterol via propionyl-CoA to the tricarboxylic acid cycle. MCM has different functions in other species.

References & Citations

1. Adjalla, C. E., Hosack, A. R., Gilfix, B. M., Lamothe, E., Sun, S., Chan, A., Evans, S., Matiaszuk, N. V., Rosenblatt, D. S. Seven novel mutations in mut methylmalonic aciduria. Hum. Mutat. 11: 270-274, 1998. 2. Berger, I., Shaag, A., Anikster, Y., Baumgartner, E. R., Bar-Meir, M., Joseph, A., Elpeleg, O. N. Mutation analysis of the MCM gene in Israeli patients with mut (0) disease. Molec. Genet. Metab. 73: 107-110, 2001. 3. Cavicchi, C., Donati, M. A., Funghini, S., la Marca, G., Malvagia, S., Ciani, F., Poggi, G. M., Pasquini, E., Zammarchi, E., Morrone, A. Genetic and biochemical approach to early prenatal diagnosis in a family with mut methylmalonic aciduria. Clin. Genet. 69: 72-76, 2006.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

83 kDa

Observed Molecular Weight

83 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Methylmalonyl-CoA mutase, mitochondrial

Subcellular Location

Mitochondrion matrix.

Protein Name

Methylmalonyl-CoA mutase, mitochondrial

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.