Anti-CD166/ALCAM Antibody Picoband®

About this product

Boster Bio Anti-CD166/ALCAM Antibody Picoband® catalog # A01788-1. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01788-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.

Synonyms

CD166 antigen; Activated leukocyte cell adhesion molecule; CD166; ALCAM; MEMD

Gene Name

ALCAM

Gene ID

214

UniProt

Q13740

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human CD166/ALCAM recombinant protein (Position: N167-E406) . Human CD166/ALCAM shares 90.8% amino acid (aa) sequence identity with both mouse and rat CD166/ALCAM.

Clonality

Polyclonal

Tissue Specificity

Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines. .

Applications

IF, IHC, WB, Flow Cytometry

Field of Research

Adaptive Immunity, B Cells, Immunology, T Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.

References & Citations

1. Bowen MA, Patel DD, Li X, Modrell B, Malacko AR, Wang WC, Marquardt H, Neubauer M, Pesando JM, Francke U, et al. (Jun 1995) . Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand. J Exp Med. 181 (6) : 2213–20. 2. Fujiwara H, Tatsumi K, Kosaka K, et al. (2003) . Human blastocysts and endometrial epithelial cells express activated leukocyte cell adhesion molecule (ALCAM/CD166) ..J. Clin. Endocrinol. Metab.88 (7) : 3437–43.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

65102 MW

Observed Molecular Weight

100-110 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

CD166 antigen

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Name

CD166 antigen

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.