Anti-ALDH1A3 Antibody Picoband®

About this product

Boster Bio Anti-ALDH1A3 Antibody Picoband® catalog # A03030. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03030

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Aldehyde dehydrogenase 1 family, member A3, also known as ALDH1A3 or retinaldehyde dehydrogenase 3 (RALDH3), is an enzyme that in humans is encoded by the ALDH1A3 gene. Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or a cellular retinol-binding protein form. Mutations in this gene have been associated with microphthalmia, isolated 8, and expression changes have also been detected in tumor cells. Alternative splicing results in multiple transcript variants.

Synonyms

Aldehyde dehydrogenase family 1 member A3;1.2.1.5; Aldehyde dehydrogenase 6; Retinaldehyde dehydrogenase 3; RALDH-3; RalDH3; ALDH1A3; ALDH6

Gene Name

ALDH1A3

Gene ID

220

UniProt

P47895

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human ALDH1A3 recombinant protein (Position: N37-K154) . Human ALDH1A3 shares 89% and 87.3% amino acid (aa) sequence identity with mouse and rat ALDH1A3, respectively.

Clonality

Polyclonal

Tissue Specificity

Expressed at low levels in many tissues and at higher levels in salivary gland, stomach, and kidney.

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cofactors, Vitamins/Minerals, Metabolic Signaling Pathways, Metabolism, Neuroscience, Olfactory System, Pathways and Processes, Sensory System, Signal Transduction, Visual System, Vitamins/Minerals

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Seems to be the key enzyme in the formation of an RA gradient along the dorso-ventral axis during the early eye development and also in the development of the olfactory system (By similarity) . .

References & Citations

1. Nishimura M, Yoshitsugu H, Naito S, Hiraoka I (2002) . Evaluation of gene induction of drug-metabolizing enzymes and transporters in primary culture of human hepatocytes using high-sensitivity real-time reverse transcription PCR.. Yakugaku Zasshi. 122 (5) : 339–61. 2. Rexer BN, Zheng WL, Ong DE (2001) . Retinoic acid biosynthesis by normal human breast epithelium is via aldehyde dehydrogenase 6, absent in MCF-7 cells.. Cancer Res. 61 (19) : 7065–70. 3. Yoshida A, Rzhetsky A, Hsu LC, Chang C (1998) . Human aldehyde dehydrogenase gene family.. Eur. J. Biochem. 251 (3) : 549–57.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

56 kDa

Observed Molecular Weight

50-56 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Aldehyde dehydrogenase family 1 member A3

Subcellular Location

Cytoplasm .

Protein Name

Aldehyde dehydrogenase family 1 member A3

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.