Anti-ECE1 Antibody Picoband®

About this product

Boster Bio Anti-ECE1 Antibody Picoband® catalog # A02519-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02519-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Endothelin converting enzyme 1, also known as ECE1, is an enzyme which in humans is encoded by the ECE1 gene. The protein encoded by this gene is involved in proteolytic processing of endothelin precursors to biologically active peptides. Mutations in this gene are associated with Hirschsprung disease, cardiac defects and autonomic dysfunction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Synonyms

Endothelin-converting enzyme 1; ECE-1;3.4.24.71; ECE1

Gene Name

ECE1

Gene ID

1889

UniProt

P42892

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human ECE1 recombinant protein (Position: M18-T233) . Human ECE1 shares 94% and 93.5% amino acid (aa) sequence identity with mouse and rat ECE1, respectively.

Clonality

Polyclonal

Tissue Specificity

All isoforms are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma; isoforms A and C in liver, testis and small intestine; isoform B, C and D in endothelial cells and umbilical vein smooth muscle cells; isoforms C and D in saphenous vein cells, and isoform C in kidney. .

Applications

WB, IHC, Flow Cytometry

Field of Research

Cancer, Cancer Metabolism, Cardiovascular, Growth Factors/Hormones, Metabolism, Metabolism Processes, Pathways and Processes, Response To Hypoxia, Signal Transduction, Vasculature

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Converts big endothelin-1 to endothelin-1. .

References & Citations

1. Schmidt M, Kröger B, Jacob E, Seulberger H, Subkowski T, Otter R, Meyer T, Schmalzing G, Hillen H (December 1994) . Molecular characterization of human and bovine endothelin converting enzyme (ECE-1) . FEBS Lett. 356 (2–3) : 238–43. 2. Entrez Gene: ECE1 endothelin converting enzyme 1.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

87164 MW

Observed Molecular Weight

87 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Endothelin-converting enzyme 1

Subcellular Location

Cell membrane; Single-pass type II membrane protein.

Protein Name

Endothelin-converting enzyme 1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.