Anti-Aquaporin 3/Aqp3 Antibody Picoband®

About this product

Boster Bio Anti-Aquaporin 3/Aqp3 Antibody Picoband® catalog # A02181. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02181

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Synonyms

Aquaporin-3; AQP-3; Aquaglyceroporin-3; Aqp3

Gene Name

Aqp3

Gene ID

11828

UniProt

Q8R2N1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of mouse Aquaporin 3, different from the related human sequence by three amino acids, and from the related rat sequence by two amino acids.

Clonality

Polyclonal

Tissue Specificity

Renal medulla and colon. Predominantly in the inner medulla. Expressed in basal layer of epidermal keratinocytes.

Applications

WB

Field of Research

Cancer, Channels, Metabolism, Plasma Membrane, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the ion of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism. .

References & Citations

1. Dibas AI, Mia AJ, Yorio T (1998) . Aquaporins (water channels) : role in vasopressin-activated water transport. Proc. Soc. Exp. Biol. Med. 219 (3) : 183–99. 2. Roudier N, Ripoche P, Gane P, Le Pennec PY, Daniels G, Cartron JP, Bailly P (2002) . AQP3 deficiency in humans and the molecular basis of a novel blood group system, GIL. J. Biol. Chem. 277 (48) : 45854–9. 3. Sasaki S, Ishibashi K, Marumo F (1998) . Aquaporin-2 and -3: representatives of two subgroups of the aquaporin family colocalized in the kidney collecting duct. Annu. Rev. Physiol. 60: 199–220.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

31568 MW

Observed Molecular Weight

32 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Aquaporin-3

Subcellular Location

Membrane; Multi-pass membrane protein.

Protein Name

Aquaporin-3

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.