- Catalog number
- A07297-1
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Gamma-aminobutyric acid (GABA) B receptor, 1 (GABAB1), is a G-protein coupled receptor subunit encoded by the GABBR1 gene. This gene encodes a receptor for gamma-aminobutyric acid (GABA), which is the main inhibitory neurotransmitter in the mammalian central nervous system. This receptor functions as a heterodimer with GABA (B) receptor 2. Defects in this gene may underlie brain disorders such as schizophrenia and epilepsy. Alternative splicing generates multiple transcript variants, but the full-length nature of some of these variants has not been determined.
- Synonyms
- Gamma-aminobutyric acid type B receptor subunit 1; GABA-B receptor 1; GABA-B-R1; GABA-BR1; GABABR1; Gb1; GABBR1; GPRC3A
- Gene Name
- GABBR1
- Gene ID
- 2550
- UniProt
- Q9UBS5
- Host
- Rabbit
- Reactivity
- Human, Mouse, Rat
- Cross Reactivity
- No cross-reactivity with other proteins.
- Immunogen
- E.coli-derived human GABBR1 recombinant protein (Position: Q186-D405) . Human GABBR1 shares 99.5% amino acid (aa) sequence identity with both mouse and rat GABBR1.
- Clonality
- Polyclonal
- Tissue Specificity
- Highly expressed in brain and weakly in heart, small intestine and uterus. Isoform 1A is mostly expressed in granular cell and molecular layer. Isoform 1B is mostly expressed in Purkinje cells. Isoform 1E is predominantly expressed in peripheral tissues as kidney, lung, trachea, colon, small intestine, stomach, bone marrow, thymus and mammary gland. .
- Applications
- WB
- Field of Research
- Interleukins
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Calcium is required for high affinity binding to GABA. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. Activated by (-) -baclofen, cgp27492 and blocked by phaclofen.
- References & Citations
- 1. White JH, McIllhinney RA, Wise A, Ciruela F, Chan WY, Emson PC, Billinton A, Marshall FH (December 2000) . The GABAB receptor interacts ly with the related transcription factors CREB2 and ATFx. Proc. Natl. Acad. Sci. U.S.A. 97 (25) : 13967–72. 2. White JH, Wise A, Main MJ, Green A, Fraser NJ, Disney GH, Barnes AA, Emson P, Foord SM, Marshall FH (December 1998) . Heterodimerization is required for the formation of a functional GABA (B) receptor. Nature. 396 (6712) : 679–82.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 108320 MW
- Observed Molecular Weight
- 108 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Gamma-aminobutyric acid type B receptor subunit 1
- Subcellular Location
- Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell projection, dendrite . Colocalizes with ATF4 in hippocampal neuron dendritic membranes (By similarity) . Coexpression of GABBR1 and GABBR2 is required for GABBR1 maturation and transport to the plasma membrane. .
- Protein Name
- Gamma-aminobutyric acid type B receptor subunit 1
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
