Anti-GDF15 Antibody Picoband®

About this product

Boster Bio Anti-GDF15 Antibody Picoband® catalog # A01583-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01583-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Growth/differentiation factor 15 (GDF15) is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF15 is also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB. GDF15 mRNA is most abundant in the liver, with lower levels seen in some other tissues. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung.

Synonyms

Growth/differentiation factor 15; GDF-15; Macrophage inhibitory cytokine 1; MIC-1; NSAID-activated gene 1 protein; NAG-1; NSAID-regulated gene 1 protein; NRG-1; Placental TGF-beta; Placental bone morphogenetic protein; Prostate differentiation factor; GDF15; MIC1, PDF, PLAB, PTGFB

Gene Name

GDF15

Gene ID

9518

UniProt

Q99988

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human GDF15 recombinant protein (Position: A195-I308) . Human GDF15 shares 68.1% and 69.2% amino acid (aa) sequence identity with mouse and rat GDF15, respectively.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney.

Applications

ELISA (Cap), WB

Field of Research

Cardiovascular, Cell Biology, Cell Cycle, Cell Division, Cytokinesis, Cytoskeleton/ECM, Extracellular Matrix, Growth Factors/Hormones, Hypertrophy, Signal Transduction, Structures

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the lysosomal compartment. Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. May bind and mobilize cholesterol that is associated with membranes. NPC2 binds cholesterol with a 1:1 stoichiometry. Can bind a variety of sterols, including lathosterol, desmosterol and the plant sterols stigmasterol and beta-sitosterol. The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport.

References & Citations

1. Hsiao E, Koniaris L, Zimmers-Koniaris T, Sebald S, Huynh T, Lee S (2000) . Characterization of growth-differentiation factor 15, a transforming growth factor beta superfamily member induced following liver injury. Mol Cell Biol. 20 (10) : 3742–3751. 2. Zimmers T, Jin X, Hsiao E, McGrath S, Esquela A, Koniaris L (2005) . Growth differentiation factor-15/macrophage inhibitory cytokine-1 induction after kidney and lung injury. Shock. 23 (6) : 543–8.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

34140 MW

Observed Molecular Weight

34 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Growth/differentiation factor 15

Subcellular Location

Secreted .

Protein Name

Growth/differentiation factor 15

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.