Anti-ATF6 Antibody Picoband®

About this product

Boster Bio Anti-ATF6 Antibody Picoband® catalog # A00655. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00655

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

ATF6, a member of the leucine zipper protein family, is an endoplasmic reticulum (ER) stress-regulated transmembrane transcription factor that activates the transcription of ER molecules. This gene is mapped to chromosome 1q23.3. ATF6 can constitutively induce the promoter of glucose-regulated protein (grp) genes through activation of the endoplasmic reticulum (ER) stress element (ERSE) .

Synonyms

Cyclic AMP-dependent transcription factor ATF-6 alpha; cAMP-dependent transcription factor ATF-6 alpha; Activating transcription factor 6 alpha; ATF6-alpha; Processed cyclic AMP-dependent transcription factor ATF-6 alpha; ATF6

Gene Name

ATF6

Gene ID

22926

UniProt

P18850

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ATF6, different from the related mouse and rat sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous.

Applications

IHC, WB

Field of Research

2339, Domain Families, Epigenetics and Nuclear Signaling, Hlh/Leucine Zipper, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N (9) -CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3') . Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. .

References & Citations

1. Li, M.; Baumeister, P.; Roy, B.; Phan, T.; Foti, D.; Luo, S.; Lee, A. S. : ATF6 as a transcription activator of the endoplasmic reticulum stress element: thapsigargin stress-induced changes and synergistic interactions with NF-Y and YY1. Molec. Cell. Biol. 20: 5096-5106, 2000. 2. Thameem, F.; Farook, V. S.; Bogardus, C.; Prochazka, M. : Association of amino acid variants in the activating transcription factor 6 gene (ATF6) on 1q21-q23 with type 2 diabetes in Pima Indians. Diabetes 55: 839-842, 2006.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

74585 MW

Observed Molecular Weight

90 kDa, 100 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Cyclic AMP-dependent transcription factor ATF-6 alpha

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type II membrane protein.

Protein Name

Cyclic AMP-dependent transcription factor ATF-6 alpha

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.