Anti-CPI17 alpha/PPP1R14A Antibody Picoband®

About this product

Boster Bio Anti-CPI17 alpha/PPP1R14A Antibody Picoband® catalog # A05752-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A05752-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protein phosphatase 1 regulatory subunit 14A, also known as CPI-17, is a protein that in humans is encoded by the PPP1R14A gene. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Synonyms

Protein phosphatase 1 regulatory subunit 14A;17 kDa PKC-potentiated inhibitory protein of PP1; Protein kinase C-potentiated inhibitor protein of 17 kDa; CPI-17; PPP1R14A; CPI17, PPP1INL

Gene Name

PPP1R14A

Gene ID

94274

UniProt

Q96A00

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human CPI17 alpha recombinant protein (Position: L30-Q126) . Human CPI17 alpha shares 83.5% and 84.5% amino acid (aa) sequence identity with mouse and rat CPI17 alpha, respectively.

Clonality

Polyclonal

Tissue Specificity

Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta. .

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.

References & Citations

1. Eto M, Ohmori T, Suzuki M, et al. (1996) . A novel protein phosphatase-1 inhibitory protein potentiated by protein kinase C. Isolation from porcine aorta media and characterization.. J. Biochem. 118 (6) : 1104–7. 2. Yamawaki K, Ito M, Machida H, Moriki N, Okamoto R, Isaka N, Shimpo H, Kohda A, Okumura K, Hartshorne DJ, Nakano T (Jul 2001) . Identification of human CPI-17, an inhibitory phosphoprotein for myosin phosphatase. Biochem Biophys Res Commun. 285 (4) : 1040–5.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

16693 MW

Observed Molecular Weight

20 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Protein phosphatase 1 regulatory subunit 14A

Subcellular Location

Cytoplasm .

Protein Name

Protein phosphatase 1 regulatory subunit 14A

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.