Anti-Bag1 Antibody Picoband®

About this product

Boster Bio Anti-Bag1 Antibody Picoband® catalog # A02423-2. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02423-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

BAG family molecular chaperone regulator 1 (BAG1) is a protein that in humans is encoded by the BAG1 gene. Human BAG1 is mapped to chromosome 9p12, a region associated with hereditary disorders that may involve developmental dysregulation of programmed cell death. The Bag1 protein is rich in glutamic acid residues. Its deduced 274-amino acid protein has a calculated molecular mass of 31 KD. Being the BCL-2-associated athanogene, Bag1 enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms.

Synonyms

BAG family molecular chaperone regulator 1; BAG-1; Bcl-2-associated athanogene 1; BAG1; HAP

Gene Name

BAG1

Gene ID

573

UniProt

Q99933

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Bag1, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level) . .

Applications

IHC, WB

Field of Research

Apoptosis, Apoptotic Markers, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cell Death, Intracellular, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli. .

References & Citations

1. Takayama S, Sato T, Krajewski S, Kochel K, Irie S, Millan JA, Reed JC (Mar 1995) . Cloning and functional analysis of BAG-1: a novel Bcl-2-binding protein with anti-cell death activity. Cell 80 (2) : 279–84. 2. Takayama, S., Kochel, K., Irie, S., Inazawa, J., Abe, T., Sato, T., Druck, T., Huebner, K., Reed, J. C.Cloning of cDNAs encoding the human BAG1 protein and localization of the human BAG1 gene to chromosome 9p12.Genomics35: 494-498, 1996. 3. Entrez Gene: BAG1 BCL2-associated athanogene.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

38779 MW

Observed Molecular Weight

33, 46, 56 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

BAG family molecular chaperone regulator 1

Subcellular Location

Isoform 1: Nucleus. Cytoplasm. Isoform 1 localizes predominantly to the nucleus.

Protein Name

BAG family molecular chaperone regulator 1

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.