Anti-Hydroxysteroid (17-beta) Dehydrogenase 4/HSD17B4 Antibody Picoband®

About this product

Boster Bio Anti-Hydroxysteroid (17-beta) Dehydrogenase 4/HSD17B4 Antibody Picoband® catalog # A02923. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02923

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Peroxisomal multifunctional enzyme type 2 is a protein that in humans is encoded by the HSD17B4 gene. The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD) . An apparent pseudogene of this gene is present on chromosome 8. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Synonyms

Peroxisomal multifunctional enzyme type 2; MFE-2;17-beta-hydroxysteroid dehydrogenase 4;17-beta-HSD 4; D-bifunctional protein; DBP; Multifunctional protein 2; MPF-2; Short chain dehydrogenase/reductase family 8C member 1; (3R) -hydroxyacyl-CoA dehydrogenase;1.1.1.n12; Enoyl-CoA hydratase 2;4.2.1.107;4.2.1.119;3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase; HSD17B4; EDH17B4, SDR8C1

Gene Name

HSD17B4

Gene ID

3295

UniProt

P51659

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human HSD17B4 recombinant protein (Position: D510-L736) . Human HSD17B4 shares 87.7% and 89% amino acid (aa) sequence identity with mouse and rat HSD17B4, respectively.

Clonality

Polyclonal

Tissue Specificity

Present in many tissues with highest concentrations in liver, heart, prostate and testis.

Applications

IHC, WB

Field of Research

Cancer, Cardiovascular, Fatty Acid Oxidation, Fatty Acids, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Redox Metabolism, Signal Transduction, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Bifunctional enzyme acting on the peroxisomal beta- oxidation pathway for fatty acids. Catalyzes the formation of 3- ketoacyl-CoA intermediates from both straight-chain and 2-methyl- branched-chain fatty acids. .

References & Citations

1. Entrez Gene: HSD17B4 Hydroxysteroid (17-beta) dehydrogenase 1. 2. Aka JA, Mazumdar M, Chen CQ, Poirier D, Lin SX (Apr 2010) . 17beta-hydroxysteroid dehydrogenase type 1 stimulates breast cancer by dihydrotestosterone inactivation in addition to estradiol production.Molecular Endocrinology. 24 (4) : 832–45.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

79686 MW

Observed Molecular Weight

80 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Peroxisomal multifunctional enzyme type 2

Subcellular Location

Peroxisome.

Protein Name

Peroxisomal multifunctional enzyme type 2

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.