Anti-HSPB8/Hsp22 Antibody Picoband®

About this product

Boster Bio Anti-HSPB8/Hsp22 Antibody Picoband® catalog # A02492-2. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02492-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Heat shock protein beta-8 is a protein that in humans is encoded by the HSPB8 gene. The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.

Synonyms

Heat shock protein beta-8; HspB8; Alpha-crystallin C chain; E2-induced gene 1 protein; Protein kinase H11; Small stress protein-like protein HSP22; HSPB8; CRYAC, E2IG1, HSP22; PP1629

Gene Name

HSPB8

Gene ID

26353

UniProt

Q9UJY1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human HSPB8/Hsp22 recombinant protein (Position: M1-T196) . Human HSPB8/Hsp22 shares 94.4% and 95.4% amino acid (aa) sequence identity with mouse and rat HSPB8/Hsp22, respectively.

Clonality

Polyclonal

Tissue Specificity

Predominantly expressed in skeletal muscle and heart. .

Applications

Flow Cytometry, IP, IF, IHC, ICC, WB

Field of Research

Chaperones, Heat Shock Proteins, Neurodegenerative Disease, Neurology Process, Neuroscience, Protein Trafficking, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Displays temperature-dependent chaperone activity.

References & Citations

1. Smith CC, Yu YX, Kulka M, Aurelian L (Sep 2000) . A novel human gene similar to the protein kinase (PK) coding domain of the large subunit of herpes simplex virus type 2 ribonucleotide reductase (ICP10) codes for a serine-threonine PK and is expressed in melanoma cells. J Biol Chem. 275 (33) : 25690–9. 2. Entrez Gene: HSPB8 Heat shock 22kDa protein 8.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

21604 MW

Observed Molecular Weight

22 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Heat shock protein beta-8

Subcellular Location

Cytoplasm . Nucleus . Translocates to nuclear foci during heat shock.

Protein Name

Heat shock protein beta-8

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.