Anti-Thrombospondin/THBS1 Antibody Picoband®

About this product

Boster Bio Anti-Thrombospondin/THBS1 Antibody Picoband® catalog # A00667-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00667-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

THBS1 is also known as Thrombospondin 1, TSP1. The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. It is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. It has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. In addition, the thrombospondin-1 protein is a member of the thrombospondinfamily. It is a multi-domain matrix glycoprotein that has been shown to be a natural inhibitor of neovascularization and tumorigenesis in healthy tissue. Both positive and negative modulation of endothelial cell adhesion, motility, and growth have been attributed to TSP1. This should not be surprising considering that TSP1 interacts with at least 12 cell adhesion receptors, including CD36, αv integrins, β1 integrins, syndecan, and integrin-associated protein (IAP or CD47) . It also interacts with numerous proteases involved in angiogenesis, including plasminogen, urokinase, matrix metalloproteinase, thrombin, cathepsin, and elastase.

Synonyms

Thrombospondin-1; THBS1; TSP, TSP1

Gene Name

THBS1

Gene ID

7057

UniProt

P07996

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human Thrombospondin recombinant protein (Position: D27-H112) . Human Thrombospondin shares 81.4% amino acid (aa) sequence identity with mouse Thrombospondin.

Clonality

Polyclonal

Tissue Specificity

Expressed in natural killer (NK) cells, CD8 (+) alpha-beta and gamma-delta T-cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Expressed in interferon-producing killer dendritic cells (IKDCs) .

Applications

ELISA, WB

Field of Research

Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Cell Adhesion Proteins, Cell Type Marker, Invasion/Microenvironment, Metabolism, Metabolism Processes, Neuroscience, Pathways and Processes, Platelets, Response To Hypoxia, Secreted, Signaling Pathways, Stem Cells, TGF Beta

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity) . Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity) . .

References & Citations

1. Blake SM, Strasser V, Andrade N et al. (October 2008) . Thrombospondin-1 binds to ApoER2 and VLDL receptor and functions in postnatal neuronal migration. The EMBO Journal 27 (22) : 3069–80. 2. Lopez-Dee ZP, Chittur SV, Patel B, Stanton R, Wakeley M, Lippert B, Menaker A, Eiche B, Terry R, Gutierrez LS (2012) .Thrombospondin-1 type 1 repeats in a model of inflammatory bowel disease: transcript profile and therapeutic effects. PLoS ONE7 (4) : e34590. 3. Simantov R, Silverstein RL (September 2003) . CD36: a critical anti-angiogenic receptor. Frontiers in bioscience : a journal and virtual library 8 (1-3) : s874–82.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

129383 MW

Observed Molecular Weight

165 kDa, 180 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Thrombospondin-1

Subcellular Location

Endoplasmic reticulum . Sarcoplasmic reticulum .

Protein Name

Thrombospondin-1

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.