Anti-NCKAP1 Antibody Picoband®

About this product

Boster Bio Anti-NCKAP1 Antibody Picoband® catalog # A09238-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A09238-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Nck-associated protein 1 is a protein that in humans is encoded by the NCKAP1 gene. By somatic cell hybrid analysis, the NCKAP1 gene was mapped to chromosome 2. NCKAP1 is a predicted type II transmembrane protein, with a C-terminal transmembrane domain. It was demonstrated that suppression of NCKAP1 expression using an antisense NCKAP1 oligonucleotide induced apoptosis of neuronal cells.

Synonyms

Nck-associated protein 1; NAP 1; Membrane-associated protein HEM-2; p125Nap1; NCKAP1; HEM2, KIAA0587, NAP1

Gene Name

NCKAP1

Gene ID

10787

UniProt

Q9Y2A7

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NCKAP1 recombinant protein (Position: S2-K201) . Human NCKAP1 shares 98.5% and 98% amino acid (aa) sequence identity with mouse and rat NCKAP1, respectively.

Clonality

Polyclonal

Tissue Specificity

Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle.

Applications

WB

Field of Research

Neurogenesis, Neurology Process, Neuroscience, NFKB Pathway, Nuclear Signaling, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1.

References & Citations

1. Eden S, Rohatgi R, Podtelejnikov AV, Mann M, Kirschner MW (Aug 2002) . Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck. Nature. 418 (6899) : 790–3. 2. Kitamura Y, Kitamura T, Sakaue H, Maeda T, Ueno H, Nishio S, Ohno S, Osada SI, Sakaue M, Ogawa W, Kasuga M (Mar 1997) . Interaction of Nck-associated protein 1 with activated GTP-binding protein Rac. The Biochemical Journal. 322 (Pt 3) : 873–8. 3. Suzuki, T., Nishiyama, K., Yamamoto, A., Inazawa, J., Iwaki, T., Yamada, T., Kanazawa, I., Sakaki, Y. Molecular cloning of a novel apoptosis-related gene, human Nap1 (NCKAP1), and its possible relation to Alzheimer disease. Genomics 63: 246-254, 2000.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

128790 MW

Observed Molecular Weight

129 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Nck-associated protein 1

Subcellular Location

Cell membrane ; Single-pass membrane protein ; Cytoplasmic side . Cell projection, lamellipodium membrane ; Single-pass membrane protein ; Cytoplasmic side . At the interface between the lamellipodial actin meshwork and the membrane. .

Protein Name

Nck-associated protein 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.