Anti-Carbonic Anhydrase IV/CA4 Antibody Picoband®

About this product

Boster Bio Anti-Carbonic Anhydrase IV/CA4 Antibody Picoband® catalog # A01523-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01523-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Carbonic anhydrase 4 is an enzyme that in humans is encoded by the CA4 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport.

Synonyms

Carbonic anhydrase 4;4.2.1.1; Carbonate dehydratase IV; Carbonic anhydrase IV; CA-IV; CA4

Gene Name

CA4

Gene ID

762

UniProt

P22748

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CA IV recombinant protein (Position: A19-S284) . Human CA IV shares 55.5% and 58.9% amino acid (aa) sequence identity with mouse and rat CA IV, respectively.

Clonality

Polyclonal

Tissue Specificity

Expressed in the endothelium of the choriocapillaris in eyes (at protein level) . Not expressed in the retinal epithelium at detectable levels. .

Applications

WB

Field of Research

Neuroscience, Sensory System, Visual System

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid. .

References & Citations

1. Okuyama T, Batanian JR, Sly WS (Aug 1993) . Genomic organization and localization of gene for human carbonic anhydrase IV to chromosome 17q. Genomics. 16 (3) : 678–84. 2. Sterling D, Alvarez BV, Casey JR (Jul 2002) . The extracellular component of a transport metabolon. Extracellular loop 4 of the human AE1 Cl-/HCO3- exchanger binds carbonic anhydrase IV. J. Biol. Chem. 277 (28) : 25239–46.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

35032 MW

Observed Molecular Weight

35 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Carbonic anhydrase 4

Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor.

Protein Name

Carbonic anhydrase 4

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.