Anti-CHD2 Antibody Picoband®

About this product

Boster Bio Anti-CHD2 Antibody Picoband® catalog # A04079. Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A04079

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Chromodomain-helicase-DNA-binding protein 2 is an enzyme that in humans is encoded by the CHD2 gene. The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. CHD2 catalyzes the assembly of chromatin into periodic arrays; and the N-terminal region of CHD2, which contains tandem chromodomains, serves an auto-inhibitory role in both the DNA-binding and ATPase activities of CHD2.

Synonyms

Chromodomain-helicase-DNA-binding protein 2; CHD-2;3.6.4.12; ATP-dependent helicase CHD2; CHD2

Gene Name

CHD2

Gene ID

1106

UniProt

O14647

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human CHD2 recombinant protein (Position: R1124-K1351) . Human CHD2 shares 97.4% amino acid (aa) sequence identity with mouse CHD2.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Expressed in sweat glands and bronchiolar epithelium (PubMed:26911677) .

Applications

IHC, WB

Field of Research

Chromatin Remodeling, Epigenetics and Nuclear Signaling, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

DNA-binding helicase that specifically binds to the promoter of target genes, leading to chromatin remodeling, possibly by promoting deposition of histone H3.3. Involved in myogenesis via interaction with MYOD1: binds to myogenic gene regulatory sequences and mediates incorporation of histone H3.3 prior to the onset of myogenic gene expression, promoting their expression (By similarity) . .

References & Citations

1. Entrez Gene: CHD2 chromodomain helicase DNA binding protein 2. 2. Liu JC, Ferreira CG, Yusufzai T (Jan 2015) . Human CHD2 is a chromatin assembly ATPase regulated by its chromo- and DNA-binding domains. The Journal of Biological Chemistry. 290 (1) : 25–34. 3. Woodage T, Basrai MA, Baxevanis AD, Hieter P, Collins FS (Oct 1997) . Characterization of the CHD family of proteins. Proceedings of the National Academy of Sciences of the United States of America. 94 (21) : 11472–7.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

211344 MW

Observed Molecular Weight

250 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Chromodomain-helicase-DNA-binding protein 2

Subcellular Location

Nucleus . Binds to myogenic gene promoters. .

Protein Name

Acid-sensing ion channel 2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.