Anti-Hsp20/HSPB6 Antibody Picoband®

About this product

Boster Bio Anti-Hsp20/HSPB6 Antibody Picoband® catalog # A07981-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A07981-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Heat shock protein beta-6 is a protein that in humans is encoded by the HSPB6 gene. HSPB6, also known as hsp20, is a 17-kDa member of the small heat shock family of proteins. It is expressed in multiple tissues; however, HSPB6 is most highly and constitutively expressed in different types of muscle including vascular, airway, colonic, bladder, uterine smooth muscle, cardiac muscle and skeletal muscle. HSPB6 has specific functions for vasodilation, platelet function, and insulin resistance and in smooth and cardiac muscle.

Synonyms

Heat shock protein beta-6; HspB6; Heat shock 20 kDa-like protein p20; HSPB6

Gene Name

HSPB6

Gene ID

126393

UniProt

O14558

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human Hsp20 recombinant protein (Position: H73-L123) . Human Hsp20 shares 88.2% and 92.2% amino acid (aa) sequence identity with mouse and rat Hsp20, respectively.

Clonality

Polyclonal

Tissue Specificity

Mainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas.

Applications

IF, IHC, ICC, WB

Field of Research

Atherosclerosis, Cardiovascular, Chaperones, Heat Shock Proteins, Protein Trafficking, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding- competent state. Seems to have versatile functions in various biological processes. Plays a role in regulating muscle function such as smooth muscle vasorelaxation and cardiac myocyte contractility. May regulate myocardial angiogenesis implicating KDR. Overexpression mediates cardioprotection and angiogenesis after induced damage. Stabilizes monomeric YWHAZ thereby supporting YWHAZ chaperone-like activity.

References & Citations

1. Entrez Gene: HSPB6 heat shock protein, alpha-crystallin-related, B6. 2. Bukach OV, Seit-Nebi AS, Marston SB, Gusev NB (Jan 2004) . Some properties of human small heat shock protein Hsp20 (HspB6) . Eur J Biochem. 271 (2) : 291–302. 3. Dreiza CM, Komalavilas P, Furnish EJ, Flynn CR, Sheller MR, Smoke CC, Lopes LB, Brophy CM (Jan 2010) .The small heat shock protein, HSPB6, in muscle function and disease. Cell Stress Chaperones. 15 (1) : 1–11.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

17136 MW

Observed Molecular Weight

17 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Heat shock protein beta-6

Subcellular Location

Cytoplasm . Nucleus . Translocates to nuclear foci during heat shock.

Protein Name

Chromaffin granule amine transporter

Isotype

Rabbit IgG

Contents

243