Anti-KPNA2 Antibody Picoband®

About this product

Boster Bio Anti-KPNA2 Antibody Picoband® catalog # A01776-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01776-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Importin subunit alpha-2 is a protein that in humans is encoded by the KPNA2 gene. The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V (D) J recombination.

Synonyms

Importin subunit alpha-1; Karyopherin subunit alpha-2; RAG cohort protein 1; SRP1-alpha; KPNA2; RCH1, SRP1

Gene Name

KPNA2

Gene ID

3838

UniProt

P52292

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human KPNA2, different from the related mouse sequence by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Expressed ubiquitously.

Applications

WB

Field of Research

Immunoglobulins, Immunology, Nuclear Import/Export, Protein Trafficking, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and ly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The ionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

References & Citations

1. Cuomo CA, Kirch SA, Gyuris J, Brent R, Oettinger MA (Jun 1994) . Rch1, a protein that specifically interacts with the RAG-1 recombination-activating protein. Proceedings of the National Academy of Sciences of the United States of America. 91 (13) : 6156–60. 2. Weis K, Mattaj IW, Lamond AI (May 1995) . Identification of hSRP1 alpha as a functional receptor for nuclear localization sequences. Science. 268 (5213) : 1049–53.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

57862 MW

Observed Molecular Weight

58 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Importin subunit alpha-1

Subcellular Location

Cytoplasm . Nucleus .

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.