Anti-PDCD4 Antibody Picoband®

About this product

Boster Bio Anti-PDCD4 Antibody Picoband® catalog # A01105. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01105

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Programmed cell death protein 4 (PDCD4) is a protein that in humans is encoded by the PDCD4 gene. It is mapped to 10q25.2. This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. It also encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined.

Synonyms

Programmed cell death protein 4; Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a; PDCD4; H731

Gene Name

PDCD4

Gene ID

27250

UniProt

Q53EL6

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human PDCD4 recombinant protein (Position: E346-L446) . Human PDCD4 shares 97% and 96% amino acid (aa) sequence identity with mouse and rat PDCD4, respectively.

Clonality

Polyclonal

Tissue Specificity

Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma. .

Applications

Flow Cytometry, IHC, WB

Field of Research

Apoptosis, Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Cell Death, Epigenetics and Nuclear Signaling, Invasion/Microenvironment, Nucleus, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA (By similarity) . .

References & Citations

1. Azzoni, L; Zatsepina, O; Abebe, B; Bennett, IM; Kanakaraj, P; Perussia, B (1998) . Differential transcriptional regulation of CD161 and a novel gene, 197/15a, by IL-2, IL-15, and IL-12 in NK and T cells. Journal of immunology 161 (7) : 3493–500. 2. Kang MJ, Ahn HS, Lee JY, Matsuhashi S, Park WY (2002) . Up-regulation of PDCD4 in senescent human diploid fibroblasts. Biochemical and Biophysical Research Communications. 293 (1) : 617–21.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

51735 MW

Observed Molecular Weight

60 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Programmed cell death protein 4

Subcellular Location

Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.

Protein Name

Putative Polycomb group protein ASXL1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.