Anti-Ran Antibody Picoband®

About this product

Boster Bio Anti-Ran Antibody Picoband® catalog # A00204-1. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00204-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1) . Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy) . RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.

Synonyms

GTP-binding nuclear protein Ran; Androgen receptor-associated protein 24; GTPase Ran; Ras-like protein TC4; Ras-related nuclear protein; RAN; ARA24; OK/SW-cl.81

Gene Name

RAN

Gene ID

5901

UniProt

P62826

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human Ran recombinant protein (Position: A2-L216) . Human Ran shares 100% amino acid (aa) sequence identity with both mouse and rat Ran.

Clonality

Polyclonal

Tissue Specificity

Expressed in a variety of tissues. .

Applications

Flow Cytometry, IP, IF, IHC, ICC, WB

Field of Research

Small G Proteins, Spindle

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity) . The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2. .

References & Citations

1. Avis JM, Clarke PR (October 1996) . Ran, a GTPase involved in nuclear processes: its regulators and effectors. J. Cell. Sci. 109 (10) : 2423–7. 2. Ciciarello M, Mangiacasale R, Lavia P (August 2007) . Spatial control of mitosis by the GTPase Ran. Cell. Mol. Life Sci. 64 (15) : 1891–914. 3. Moore MS, Blobel G (May 1994) . A G protein involved in nucleocytoplasmic transport: the role of Ran. Trends Biochem. Sci. 19 (5) : 211–6.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

24423 MW

Observed Molecular Weight

24 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

GTP-binding nuclear protein Ran

Subcellular Location

Nucleus. Cytoplasm. Melanosome. Nucleus envelope . Predominantly nuclear during interphase. Becomes dispersed throughout the cytoplasm during mitosis. Colocalizes with NEMP1 at the nuclear envelope (By similarity) . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.