Anti-SRY Antibody Picoband®

About this product

Boster Bio Anti-SRY Antibody Picoband® catalog # A00614-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00614-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This intronless gene encodes a transcription factor that is a member of the high mobility group (HMG) -box family of DNA-binding proteins. This protein is the testis-determining factor (TDF), which initiates male sex determination. Mutations in this gene give rise to XY females with gonadal dysgenesis (Swyer syndrome) ; translocation of part of the Y chromosome containing this gene to the X chromosome causes XX male syndrome.

Synonyms

Sex-determining region Y protein; Testis-determining factor; SRY; TDF

Gene Name

SRY

Gene ID

6736

UniProt

Q05066

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human SRY.

Clonality

Polyclonal

Tissue Specificity

Plasma. Isoform 1 and isoform 2 are widely expressed. Isoform 1 is the predominant form in spleen. .

Applications

WB

Field of Research

Developmental Biology, Domain Families, Epigenetics and Nuclear Signaling, Histones, Reproduction, Transcription, Transcription Factors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcriptional regulator that controls a genetic switch in male development. It is necessary and sufficient for initiating male sex determination by ing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells (By similarity) . In male adult brain involved in the maintenance of motor functions of dopaminergic neurons (By similarity) . Involved in different aspects of gene regulation including promoter activation or repression (By similarity) . Promotes DNA bending. SRY HMG box recognizes DNA by partial intercalation in the minor groove. Also involved in pre-mRNA splicing. Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3'. .

References & Citations

1. Biason-Lauber A, Konrad D, Meyer M, DeBeaufort C, Schoenle EJ (May 2009) . Ovaries and female phenotype in a girl with 46, XY karyotype and mutations in the CBX2 gene. Am. J. Hum. Genet. 84 (5) : 658–63. 2. Margarit E, Coll MD, Oliva R, Gómez D, Soler A, Ballesta F (January 2000) . SRY gene transferred to the long arm of the X chromosome in a Y-positive XX true hermaphrodite. Am. J. Med. Genet. 90 (1) : 25–8. 3. Polanco JC, Koopman P (February 2007) . Sry and the hesitant beginnings of male development. Dev. Biol. 302 (1) : 13–24.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

23884 MW

Observed Molecular Weight

26 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Sex-determining region Y protein

Subcellular Location

Nucleus speckle. Cytoplasm. Colocalizes with SOX6 in speckles. Colocalizes with CAML in the nucleus. Colocalizes in the nucleus with ZNF208 isoform KRAB-O and tyrosine hydroxylase (TH) (By similarity) . .

Protein Name

Growth arrest-specific protein 6

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.