Anti-Estrogen Inducible Protein pS2/Tff1 Antibody Picoband®

About this product

Boster Bio Anti-Estrogen Inducible Protein pS2/Tff1 Antibody Picoband® catalog # A01391. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01391

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

TFF1 (Trefoil factor 1), also known as pS2, is a protein that in humans is encoded by the TFF1 gene. Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. It is found that TFF1 in normal human urine inhibited the growth of calcium oxalate crystals. Urinary TFF1 showed an inhibitory potency similar to that of nephrocalcin, and inhibition was dose dependent and inhibited by TFF1 antisera, particularly by antisera ed to the TFF1 C terminus. Concentrations and relative amounts of TFF1 in the urine of patients with idiopathic calcium oxalate kidney stones were significantly less than those found in controls. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.

Synonyms

Trefoil factor 1; Protein pS2; Tff1; Bcei, Ps2

Gene Name

Tff1

Gene ID

21784

UniProt

Q08423

Host

Rabbit

Reactivity

Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived mouse Tff1 recombinant protein (Position: Q22-F87) . Mouse Tff1 shares 67.2% and 83.6% amino acid (aa) sequence identity with human and rat Tff1, respectively.

Clonality

Polyclonal

Tissue Specificity

Strong expression found in normal gastric mucosa. Not detected in the small or large intestine nor in normal mammary gland.

Applications

ELISA (Cap), IHC, WB

Field of Research

Nuclear Hormone Receptors, Nuclear Signaling, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents.

References & Citations

1. Chutipongtanate, S., Nakagawa, Y., Sritippayawan, S., Pittayamateekul, J., Parichatikanond, P., Westley, B. R., May, F. E. B., Malasit, P., Thongboonkerd, V.Identification of human urinary trefoil factor 1 as a novel calcium oxalate crystal growth inhibitor.J. Clin. Invest. 115: 3613-3622, 2005. 2. Hanby, A. M., Poulsom, R., Singh, S., Elia, G., Jeffery, R. E., Wright, N. A.Spasmolytic polypeptide is a major antral peptide: distribution of the trefoil peptides human spasmolytic polypeptide and pS2 in the stomach.Gastroenterology 105: 1110-1116, 1993. 3. Roberts, M., Wallace, J., Jeltsch, J.-M., Berry, M.The 5-prime flanking region of the human pS2 gene mediates its transcriptional activation by estrogen in MCF-7 cells.Biochem. Biophys. Res. Commun. 151: 306-313, 1988.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

9670 MW

Observed Molecular Weight

17 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Trefoil factor 1

Subcellular Location

Secreted.

Protein Name

Hexokinase-2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.