Anti-IL33 Antibody Picoband®

About this product

Boster Bio Anti-IL33 Antibody Picoband® catalog # A00113. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00113

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin 33 (IL-33) is a protein that in humans is encoded by the IL33 gene. The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene.

Synonyms

Interleukin-33; IL-33; Interleukin-1 family member 11; IL-1F11; Nuclear factor from high endothelial venules; NF-HEV; Interleukin-33 (95-270) ; Interleukin-33 (99-270) ; Interleukin-33 (109-270) ; IL33; C9orf26, IL1F11, NFHEV

Gene Name

IL33

Gene ID

90865

UniProt

O95760

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human IL33 recombinant protein (Position: A95-T270) .

Clonality

Polyclonal

Tissue Specificity

Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.

Applications

ELISA (Cap), IHC, WB

Field of Research

Angiogenesis, Cardiovascular, Cytokines, Hypertrophy, Immunology, Innate Immunity, Interleukins

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016) . Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an alarmin, that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528) .

References & Citations

1. Alves-Filho, J. C., Sonego, F., Souto, F. O., Freitas, A., Verri, W. A., Jr., Auxiliadora-Martins, M., Basile-Filho, A., McKenzie, A. N., Xu, D., Cunha, F. Q., Liew, F. Y. Interleukin-33 attenuates sepsis by enhancing neutrophil influx to the site of infection. Nature Med. 16: 708-712, 2010. 2. Cayrol, C., Girard, J.-P. The IL-1-like cytokine IL-33 is inactivated after maturation by caspase-1. Proc. Nat. Acad. Sci. 106: 9021-9026, 2009.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

33275 MW

Observed Molecular Weight

18 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Interleukin 33

Subcellular Location

Nucleus

Protein Name

Programmed cell death 1 ligand 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.