Anti-MED13 Antibody Picoband®

About this product

Boster Bio Anti-MED13 Antibody Picoband® catalog # A04545-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A04545-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mediator complex subunit 13 is a protein that in humans is encoded by the MED13 gene. This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator.

Synonyms

Mediator of RNA polymerase II transcription subunit 13; Activator-recruited cofactor 250 kDa component; ARC250; Mediator complex subunit 13; Thyroid hormone receptor-associated protein 1; Thyroid hormone receptor-associated protein complex 240 kDa component; Trap240; Vitamin D3 receptor-interacting protein complex component DRIP250; DRIP250; MED13; ARC250, KIAA0593, THRAP1, TRAP240

Gene Name

MED13

Gene ID

9969

UniProt

Q9UHV7

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human MED13 recombinant protein (Position: L61-Y240) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitous.

Applications

ELISA, Flow Cytometry, IF, ICC, WB

Field of Research

Epigenetics and Nuclear Signaling, Mediator Complex, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

References & Citations

1. Gustafsson CM, Samuelsson T (Jul 2001) . Mediator--a universal complex in transcriptional regulation. Molecular Microbiology. 41 (1) : 1–8. 2. Tuchin DA, Erve PR, Schumer W (1976) . Prevention of endotoxin--induced irreversible platelet aggregation in vitro. Surgical Forum. 27 (62) : 22–3.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

34482 MW

Observed Molecular Weight

300 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Mediator complex subunit 13

Subcellular Location

Nucleus.

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.