Anti-Retinal S antigen/SAG Antibody Picoband®

About this product

Boster Bio Anti-Retinal S antigen/SAG Antibody Picoband® catalog # A03775. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03775

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

S-arrestin is a protein that in humans is encoded by the SAG gene. Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. S-arrestin, also known as S-antigen, is a major soluble photoreceptor protein that is involved in desensitization of the photoactivated transduction cascade. It is expressed in the retina and the pineal gland and inhibits coupling of rhodopsin to transducin in vitro. Additionally, S-arrestin is highly antigenic, and is capable of inducing experimental autoimmune uveoretinitis. Mutations in this gene have been associated with Oguchi disease, a rare autosomal recessive form of night blindness.

Synonyms

S-arrestin; 48 kDa protein; Retinal S-antigen; S-AG; Rod photoreceptor arrestin; SAG

Gene Name

SAG

Gene ID

6295

UniProt

P10523

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human Retinal S antigen recombinant protein (Position: R193-E405) .

Clonality

Polyclonal

Tissue Specificity

Retina and pineal gland.

Applications

ELISA, WB

Field of Research

DNA/RNA, Epigenetics and Nuclear Signaling, Nucleus, RNA Processing, Subcellular Markers, Tags & Cell Markers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Arrestin is one of the major proteins of the ros (retinal rod outer segments) ; it binds to photoactivated- phosphorylated rhodopsin, thereby apparently preventing the transducin-mediated activation of phosphodiesterase.

References & Citations

1. Calabrese, G., Sallese, M., Stornaiuolo, A., Stuppia, L., Palka, G., De Blasi, A. Chromosome mapping of the human arrestin (SAG), beta-arrestin 2 (ARRB2), and beta-adrenergic receptor kinase 2 (ADRBK2) genes. Genomics 23: 286-288, 1994. 2. Yamaki, K., Tsuda, M., Kikuchi, T., Chen, K.-H., Huang, K.-P., Shinohara, T. Structural organization of the human S-antigen gene: cDNA, amino acid, intron, exon, promoter, in vitro transcription, retina, and pineal gland. J. Biol. Chem. 265: 20757-20762, 1990.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

64133 MW

Observed Molecular Weight

45-55 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

S-antigen visual arrestin

Subcellular Location

Nucleus, nucleoplasm . Cytoplasm . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. These granules are not identical with P bodies or stress granules. .

Protein Name

Heterogeneous nuclear ribonucleoprotein L

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.