Anti-TRAIL/TNFSF10 Antibody Picoband®

About this product

Boster Bio Anti-TRAIL/TNFSF10 Antibody Picoband® catalog # A00466-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00466-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

TNF-related apoptosis-inducing ligand (TRAIL), is a protein functioning as a ligand that induces the process of cell death called apoptosis. TRAIL has also been designated CD253 (cluster of differentiation 253) . It is a member of Tumor Necrosis Factor Ligand Superfamily. In humans, the gene that encodes for TRAIL is located at chromosome 3q26. TRAIL has got 281 amino acid proteins. TRAIL mRNA is expressed at significant levels in most normal tissues. TRAIL binds to the death receptors DR4 (TRAIL-RI) and DR5 (TRAIL-RII) and the receptors DcR1 and DcR2, leading to activation of specific kinases and transcription of genes.

Synonyms

Tumor necrosis factor ligand superfamily member 10; Apo-2 ligand; Apo-2L; TNF-related apoptosis-inducing ligand; Protein TRAIL; CD253; TNFSF10; APO2L, TRAIL

Gene Name

TNFSF10

Gene ID

8743

UniProt

P50591

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human TRAIL recombinant protein (Position: N40-A272) .

Clonality

Polyclonal

Tissue Specificity

Widespread; most predominant in spleen, lung and prostate.

Applications

IHC, WB

Field of Research

Apoptosis, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cell Death, Invasion/Microenvironment, Receptors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

References & Citations

1. Pitti RM, Marsters SA, Ruppert S, Donahue CJ, Moore A, Ashkenazi A (May 1996) . Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family. J. Biol. Chem. 271 (22) : 12687–90. 2. Song JJ, Lee YJ (May 2008) . Differential cleavage of Mst1 by caspase-7/-3 is responsible for TRAIL-induced activation of the MAPK superfamily. Cell. Signal. 20 (5) : 892–906. 3. Wiley SR, Schooley K, Smolak PJ, Din WS, Huang CP, Nicholl JK, Sutherland GR, Smith TD, Rauch C, Smith CA (December 1995) . Identification and characterization of a new member of the TNF family that induces apoptosis. Immunity 3 (6) : 673–82.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

33477 MW

Observed Molecular Weight

35 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

TNF superfamily member 10

Subcellular Location

Membrane ; Single-pass type II membrane protein .

Protein Name

Tumor necrosis factor ligand superfamily member 10

Isotype

Rabbit IgG

Contents

243