Anti-Argininosuccinate Lyase/ASL Antibody Picoband®

About this product

Boster Bio Anti-Argininosuccinate Lyase/ASL Antibody Picoband® catalog # A00742-1. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00742-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

ASL (argininosuccinatelyase, also known as argininosuccinase) is an enzyme that catalyzes the reversible breakdown of argininosuccinate (ASA) producing the amino acid arginine and dicarboxylic acid fumarate. Located in liver cytosol, ASL is the fourth enzyme of the urea cycle and involved in the biosynthesis of arginine in all species and the production of urea in ureotelic species. Mutations in ASL, resulting low activity of the enzyme, increase levels of urea in the body and result in various side effects. The ASL gene is located on chromosome 7 between the centromere (junction of the long and short arm) and the long (q) arm at position 11.2, from base pair 64,984,963 to base pair 65,002,090.

Synonyms

Argininosuccinate lyase; ASAL;4.3.2.1; Arginosuccinase; ASL

Gene Name

ASL

Gene ID

435

UniProt

P04424

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Argininosuccinate Lyase/ASL, which shares 97.4% and 92.1% amino acid (aa) sequence identity with mouse and rat Argininosuccinate Lyase/ASL, respectively.

Clonality

Polyclonal

Applications

WB

Field of Research

Cancer, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.

References & Citations

1. Sampaleanu LM, Vallée F, Thompson GD, Howell PL (December 2001) . Three-dimensional structure of the argininosuccinatelyase frequently complementing allele Q286R. Biochemistry. 40 (51) : 15570–80. 2. Yu B, Howell PL (October 2000) . Intragenic complementation and the structure and function of argininosuccinatelyase. Cell. Mol. Life Sci. 57 (11) : 1637–51.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

51658 MW

Observed Molecular Weight

52 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Argininosuccinate lyase

Subcellular Location

Extracellular matrix.

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.