Anti-TRFP/MED20 Antibody Picoband®

About this product

Boster Bio Anti-TRFP/MED20 Antibody Picoband® catalog # A09407-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A09407-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

MED20 is a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder.

Synonyms

Mediator of RNA polymerase II transcription subunit 20; Mediator complex subunit 20; TRF-proximal protein homolog; hTRFP; MED20; TRFP

Gene Name

MED20

Gene ID

9477

UniProt

Q9H944

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human TRFP / MED20 recombinant protein (Position: M1-R212) .

Clonality

Polyclonal

Tissue Specificity

Pre-B-cells and B-cells but not terminally differentiated plasma cells.

Applications

ELISA, WB

Field of Research

Epigenetics and Nuclear Signaling, Mediator Complex, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

References & Citations

1. Sato, S., Tomomori-Sato, C., Banks, C. A. S., Parmely, T. J., Sorokina, I., Brower, C. S., Conaway, R. C., Conaway, J. W. A mammalian homolog of Drosophila melanogaster transcriptional coactivator Intersex is a subunit of the mammalian Mediator complex. J. Biol. Chem. 278: 49671-49674, 2003. 2. Xiao, H., Tao, Y., Roeder, R. G. The human homologue of Drosophila TRF-proximal protein is associated with an RNA polymerase II-SRB complex. J. Biol. Chem. 274: 3937-3940, 1999.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

23 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

CD72 molecule

Subcellular Location

Nucleus .

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.