Anti-PON1 Antibody Picoband®

About this product

Boster Bio Anti-PON1 Antibody Picoband® catalog # A00516. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00516

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Serum paraoxonase/arylesterase 1 (PON1), also known as aromatic esterase 1, is an enzyme that in humans is encoded by the PON1 gene. It is mapped to 7q21.3. This gene has esterase and more specifically paraoxonase activity. PON1 is responsible for hydrolysing organophosphate pesticides and nerve gasses. Polymorphisms in the PON1 gene significantly affect the catalytic ability of the enzyme. PON1 (paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein (HDL) . The PON1 gene is activated by PPAR-γ, which increases synthesis and release of paraoxonase 1 enzyme from the liver, reducing atherosclerosis.

Synonyms

Serum paraoxonase/arylesterase 1; PON 1; 3.1.1.2

Gene Name

Pon1

Gene ID

18979

UniProt

P52430

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived mouse PON1 recombinant protein (Position: A30-D274) .

Clonality

Polyclonal

Tissue Specificity

Plasma, liver, kidney, heart, brain, small intestine and lung. In the plasma, associated with HDL.

Applications

IHC, WB

Field of Research

Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Diabetes, Diabetes-associated, Drug Metabolism, Heart Disease, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Neurodegenerative Disease, Neurology Process, Neuroscience, Oxidative Stress, Pathways and Processes, Redox Metabolism, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification.

References & Citations

1. Costa LG, Cole TB, Vitalone A, Furlong CE (2005) . Measurement of paraoxonase (PON1) status as a potential biomarker of susceptibility to organophosphate toxicity. CLINICA CHEMICA ACTA 352 (1-2) : 37–47. 2. Khateeb J, Gantman A, Kreitenberg AJ, Aviram M, Fuhrman B (January 2010) . Paraoxonase 1 (PON1) expression in hepatocytes is upregulated by pomegranate polyphenols: a role for PPAR-gamma pathway.Atherosclerosis 208 (1) : 119–25. 3. Primo-Parmo SL, Sorenson RC, Teiber J, La Du BN (May 1996) . The human serum paraoxonase/arylesterase gene (PON1) is one member of a multigene family. Genomics 33 (3) : 498–507.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

43 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Paraoxonase 1

Subcellular Location

Secreted, extracellular space.

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.