Anti-GRK2/GRK2 Antibody Picoband®

About this product

Boster Bio Anti-GRK2/GRK2 Antibody Picoband® catalog # A01473-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01473-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Short transient receptor potential channel 3 (TrpC3) also known as transient receptor protein 3 (TRP-3) is a protein that in humans is encoded by the TRPC3 gene. The protein encoded by this gene is a membrane protein that can form a non-selective channel permeable to calcium and other cations. The encoded protein appears to be induced to form channels by a receptor tyrosine kinase-activated phosphatidylinositol second messenger system and also by depletion of intracellular calcium stores. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

Beta-adrenergic receptor kinase 1; Beta-ARK-1; G-protein coupled receptor kinase 2

Gene Name

GRK2

Gene ID

2156

UniProt

P25098

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human GRK2, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Expressed in peripheral blood leukocytes.

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

G Protein Signaling, Neuroscience, Neurotransmission, Receptors / Channels, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.

References & Citations

1. Benovic JL, DeBlasi A, Stone WC, Caron MG, Lefkowitz RJ (1989) . Beta-adrenergic receptor kinase: primary structure delineates a multigene family. Science. 246 (4927) : 235–40. 2. Pippig S, Andexinger S, Daniel K, Puzicha M, Caron MG, Lefkowitz RJ, Lohse MJ (1993) . Overexpression of beta-arrestin and beta-adrenergic receptor kinase augment desensitization of beta 2-adrenergic receptors.J. Biol. Chem. 268 (5) : 3201–8.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

96009 MW

Observed Molecular Weight

79 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

G protein-coupled receptor kinase 2

Subcellular Location

Cytoplasm.

Protein Name

Short transient receptor potential channel 3

Isotype

Rabbit IgG

Contents

243