Anti-AGRP Antibody

About this product

Boster Bio Anti-AGRP Antibody Picoband® catalog # A00272-1. Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A00272-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Agouti-related protein (AgRP), also called agouti-related peptide, is a neuropeptide produced in the brain by the AgRP/NPY neuron. In humans, the agouti-related peptide is encoded by the AGRP gene. This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity.

Synonyms

Agouti-related protein; Agrp; Agrt; Art

Gene Name

Agrp

Gene ID

11604

UniProt

P56473

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived mouse AGRP recombinant protein (Position: S82-T131) .

Clonality

Polyclonal

Tissue Specificity

Expressed in arcuate nucleus and median eminence, adrenal gland (medulla), hypothalamus, testis, and lung.

Applications

ELISA, IHC

Field of Research

Calcium Signaling, Cancer, Cardiovascular, Endocrine System, Fatty Acid Oxidation, Fatty Acids, Lipid and Lipoprotein Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Neurology Process, Neuroscience, Obesity, Pathways and Processes, Redox Metabolism, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R. Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity (By similarity) . It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner (By similarity) .

References & Citations

1. Ollmann MM, Wilson BD, Yang YK, Kerns JA, Chen Y, Gantz I, Barsh GS (Oct 1997) . Antagonism of central melanocortin receptors in vitro and in vivo by agouti-related protein. Science. 278 (5335) : 135–8. 2. Shutter JR, Graham M, Kinsey AC, Scully S, Lüthy R, Stark KL (Mar 1997) . Hypothalamic expression of ART, a novel gene related to agouti, is up-regulated in obese and diabetic mutant mice. Genes & Development.11 (5) : 593–602.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

68762 MW

Observed Molecular Weight

49 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Agouti related neuropeptide

Subcellular Location

Secreted.

Protein Name

Aryl hydrocarbon receptor nuclear translocator-like protein 1

Isotype

Rabbit IgG

Contents

243