Anti-CAD/CAD Antibody Picoband®

About this product

Boster Bio Anti-CAD/CAD Antibody Picoband® catalog # A00463-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00463-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This CAD gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms.

Synonyms

CAD protein; Glutamine-dependent carbamoyl-phosphate synthase

Gene Name

CAD

Gene ID

790

UniProt

P27708

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human CAD recombinant protein (Position: L1555-K1780) .

Clonality

Polyclonal

Applications

ELISA, WB

Field of Research

Apoptosis, Cancer, Cell Biology, Cell Cycle, Cell Death, DNA/Nucleotides, DNA/RNA, Epigenetics and Nuclear Signaling, Metabolic Signaling Pathways, Metabolism, Metabolism Processes, Nuclear, Nucleotide Metabolism, Nucleus, Pathways and Processes

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

This protein is a fusion protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase) .

References & Citations

1. Bertoni, L., Attolini, C., Simi, S., Giulotto, E. Localization of the Chinese hamster CAD gene reveals homology between human chromosome 2p and Chinese hamster 7q. Genomics 16: 779-781, 1993. 2. Chen, S., Bigner, S. H., Modrich, P. High rate of CAD gene amplification in human cells deficient in MLH1 or MSH6. Proc. Nat. Acad. Sci. 98: 13802-13807, 2001.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

270 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase

Subcellular Location

Cytoplasm.

Isotype

Rabbit IgG

Contents

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