Anti-CARS Antibody Picoband®

About this product

Boster Bio Anti-CARS Antibody Picoband® catalog # A00259-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00259-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This CARS gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants.

Synonyms

Cysteine--tRNA ligase, cytoplasmic;6.1.1.16; Cysteinyl-tRNA synthetase; CysRS; CARS

Gene Name

CARS

Gene ID

833

UniProt

P49589

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human CARS recombinant protein (Position: D510-Q748) .

Clonality

Polyclonal

Tissue Specificity

Testis-specific isoform is expressed in spermatocytes, adult testis.

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Amino Acid Metabolism, Amino Acids, Cancer, DNA/RNA, Epigenetics and Nuclear Signaling, Metabolic Signaling Pathways, Metabolism, Nucleotide Metabolism, Pathways and Processes, Signal Transduction, Translation

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm.

References & Citations

1. Cruzen, M. E., Bengtsson, U., McMahon, J., Wasmuth, J. J., Arfin, S. M. Assignment of the cysteinyl-tRNA synthetase gene (CARS) to 11p15.5. Genomics 15: 692-693, 1993. 2. Cools, J., Wlodarska, I., Somers, R., Mentens, N., Pedeutour, F., Maes, B., De Wolf-Peeters, C., Pauwels, P., Hagemeijer, A., Marynen, P. Identification of novel fusion partners of ALK, the anaplastic lymphoma kinase, in anaplastic large-cell lymphoma and inflammatory myofibroblastic tumor. Genes Chromosomes Cancer 34: 354-362, 2002.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

85473 MW

Observed Molecular Weight

85 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Cysteine--tRNA ligase, cytoplasmic

Subcellular Location

Cytoplasm.

Isotype

Rabbit IgG

Contents

243