Anti-EPO Receptor/EPOR Antibody Picoband®

About this product

Boster Bio Anti-EPO Receptor/EPOR Antibody Picoband® catalog # A00427-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00427-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The erythropoietin receptor (EpoR) is a protein that in humans is encoded by the EPOR gene. This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.

Synonyms

Erythropoietin receptor; EPO-R; EPOR

Gene Name

EPOR

Gene ID

2057

UniProt

P19235

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human EPO Receptor recombinant protein (Position: E48-E226) .

Clonality

Polyclonal

Tissue Specificity

Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early- stage erythroid progenitor cells.

Applications

ELISA, WB

Field of Research

Apoptosis, Cancer, Cell Death, Cytokines, Growth Factors, Growth Factors/Hormones, Immunology, Innate Immunity, Invasion/Microenvironment, Receptors, Signal Transduction, TNF Superfamily

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Receptor for erythropoietin. Mediates erythropoietin- induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.

References & Citations

1. Akashi, K., Traver, D., Miyamoto, T., Weissman, I. L. A clonogenic common myeloid progenitor that gives rise to all myeloid lineages. Nature 404: 193-197, 2000. 2. Becker, V., Schilling, M., Bachmann, J., Baumann, U., Raue, A., Maiwald, T., Timmer, J., Klingmuller, U. Covering a broad dynamic range: information processing at the erythropoietin receptor. Science 328: 1404-1408, 2010. 3. Budarf, M., Huebner, K., Emanuel, B., Croce, C. M., Copeland, N. G., Jenkins, N. A., D'Andrea, A. D. Assignment of the erythropoietin receptor (EPOR) gene to mouse chromosome 9 and human chromosome 19. Genomics 8: 575-578, 1990. 4. Jones, S. S., D'Andrea, A. D., Haines, L. L., Wong, G. G. Human erythropoietin receptor: cloning, expression, and biologic characterization. Blood 76: 31-35, 1990.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

55 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Erythropoietin receptor

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Isotype

Rabbit IgG

Contents

243