Anti-MUC1 Antibody Picoband®

About this product

Boster Bio Anti-MUC1 Antibody Picoband® catalog # A00187. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00187

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mucin 1, cell surface associated (MUC1) or polymorphic epithelial mucin (PEM) is a mucin encoded by the MUC1 gene in humans. This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. It is mapped to 1q22. Mucin 1 is a transmembrane mucin normally expressed on the apical borders of secretory epithelial cells. Overexpression of Mucin 1 is often associated with colon, breast, ovarian, lung and pancreatic cancers. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Mucin 1 stimulated ESR1-mediated transcription and contributed to estradiol-mediated growth and survival of breast cancer cells. This gene also can suppress pulmonary innate immunity, and its antiinflammatory activity may play an important modulatory role during microbial infection.

Synonyms

Mucin-1; MUC-1; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut-reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA; Tumor-associated mucin; CD227; Mucin-1 subunit alpha; MUC1-NT; MUC1-alpha; Mucin-1 subunit beta; MUC1-beta; MUC1-CT; MUC1; PUM

Gene Name

MUC1

Gene ID

4582

UniProt

P15941

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human MUC1 recombinant protein (Position: S1062-V1139) .

Clonality

Polyclonal

Tissue Specificity

Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform Y is expressed in tumor cells only.

Applications

ELISA, IHC, WB

Field of Research

Cancer, Cell Type Markers, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction, Tags & Cell Markers, Tumor Associated, Tumor Immunology, Tumor-Associated Antigens

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.

References & Citations

1. Gendler SJ, Lancaster CA, Taylor-Papadimitriou J, Duhig T, Peat N, Burchell J, Pemberton L, Lalani EN, Wilson D (September 1990) . Molecular cloning and expression of human tumor-associated polymorphic epithelial mucin. J. Biol. Chem. 265 (25) : 15286–93. 2. Lu, W., Hisatsune, A., Koga, T., Kato, K., Kuwahara, I., Lillehoj, E. P., Chen, W., Cross, A. S., Gendler, S. J., Gewirtz, A. T., Kim, K. C. Cutting edge: enhanced pulmonary clearance of Pseudomonas aeruginosa by Muc1 knockout mice. J. Immun. 176: 3890-3894, 2006. 3. Wei, X., Xu, H., Kufe, D. MUC1 oncoprotein stabilizes and activates estrogen receptor alpha. Molec. Cell 21: 295-305, 2006.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

122 kDa

Observed Molecular Weight

120, 200 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Mucin 1, cell surface associated

Subcellular Location

Apical cell membrane.

Protein Name

Apoptosis regulator BAX

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.