Anti-MUC2 Antibody

About this product

Anti-MUC2

Specifications

Catalog number

A01212

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mucin 2, also known as MUC2, is a protein that in humans is encoded by the MUC2 gene. This gene encodes a member of the mucin protein family. It is mapped to 11p15.5. Mucin 2 is particularly prominent in the gut where it is secreted from goblet cells in the epithelial lining into the lumen of the large intestine. There, mucin 2, along with small amounts of related-mucin proteins, polymerizes into a gel of which 80% by weight is oligosaccharide side-chains that are added as post-translational modifications to the mucin proteins. This gel provides an insoluble mucous barrier that serves to protect the intestinal epithelium. The primary function of the MUC2 gene product is to provide a protective barrier between the epithelial surfaces and the gut lumen. There is decreased expression of MUC2 in colonic cancer and defective polymerization of secreted mucin in ulcerative colitis.

Synonyms

Mucin-2; MUC-2; Intestinal mucin-2; MUC2; SMUC

Gene Name

MUC2

Gene ID

4583

UniProt

Q02817

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human MUC2, which shares 86.1% amino acid (aa) sequence identity with both mouse and rat MUC2.

Clonality

Polyclonal

Tissue Specificity

Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.

Applications

IF, IHC

Field of Research

Cancer, Cell Type Markers, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction, Tags & Cell Markers, Tumor Associated, Tumor Immunology, Tumor-Associated Antigens

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.

References & Citations

1. Allen, A., Hutton, D. A., Pearson, J. P. The MUC2 gene product: a human intestinal mucin. Int. J. Biochem. Cell Biol. 30: 797-801, 1998. 2. Rousseau K, Byrne C, Kim YS, Gum JR, Swallow DM, Toribara NW (May 2004) . The complete genomic organization of the human MUC6 and MUC2 mucin genes. Genomics 83 (5) : 936–9.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

123799 MW

Observed Molecular Weight

38 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Mucin 2, oligomeric mucus/gel-forming

Subcellular Location

Secreted. In the intestine, secreted into the inner and outer mucus layers.

Protein Name

Vinculin

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.