Anti-PF4 Antibody Picoband®

About this product

Boster Bio Anti-PF4 Antibody Picoband® catalog # A00871. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00871

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4) . By in situ hybridization, the CXCL4 gene is mapped to chromosome 4q12-q21. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.

Synonyms

Platelet factor 4; PF-4; C-X-C motif chemokine 4; Pf4; Cxcl4; Scyb4

Gene Name

Pf4

Gene ID

360918

UniProt

P06765

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived rat PF4 recombinant protein (Position: V30-S105) .

Clonality

Polyclonal

Applications

ELISA, IHC, WB

Field of Research

Atherosclerosis, Cardiovascular, Chemokines, Immunology, Innate Immunity, Macrophage/Inflammation, Platelets, Vascular Inflammation

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation.

References & Citations

1. Eisman, R., Surrey, S., Ramachandran, B., Schwartz, E., Poncz, M. Structural and functional comparison of the genes for human platelet factor 4 and PF4-alt. Blood 76: 336-344, 1990. 2. Griffin, C. A., Emanuel, B. S., LaRocco, P., Schwartz, E., Poncz, M. Human platelet factor 4 gene is mapped to 4q12-q21. Cytogenet. Cell Genet. 45: 67-69, 1987. 3. Lambert, M. P., Rauova, L., Bailey, M., Sola-Visner, M. C., Kowalska, M. A., Poncz, M. Platelet factor 4 is a negative autocrine in vivo regulator of megakaryopoiesis: clinical and therapeutic implications. Blood 110: 1153-1160, 2007.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

11 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Platelet factor 4

Subcellular Location

Secreted.

Isotype

Rabbit IgG

Contents

243