Anti-TFF3 Antibody Picoband®

About this product

Boster Bio Anti-TFF3 Antibody Picoband® catalog # A01738-3. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01738-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. It is mapped to 21q22.3. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to antiapoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies.

Synonyms

Trefoil factor 3; Intestinal trefoil factor; mITF; Tff3; Itf

Gene Name

Tff3

Gene ID

21786

UniProt

Q62395

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived mouse TFF3 recombinant protein (Position: A23-F81) .

Clonality

Polyclonal

Tissue Specificity

Expressed in goblet cells of the intestines and colon (at protein level) . Expressed abundantly in goblet cells of intestine and colon, and at low levels in stomach. No expression in brain, lung, spleen, kidney, uterus, pancreas, liver, heart or thymus.

Applications

ELISA, IF, IHC, WB

Field of Research

Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen) .

References & Citations

1. Paulsen, F. P., Woon, C.-W., Varoga, D., Jansen, A., Garreis, F., Jager, K., Amm, M., Podolsky, D. K., Steven, P., Barker, N. P., Sel, S. Intestinal trefoil factor/TFF3 promotes re-epithelialization of corneal wounds. J. Biol. Chem. 283: 13418-13427, 2008. 2. Schmitt, H., Wundrack, I., Beck, S., Gott, P., Welter, C., Shizuya, H., Simon, M. I., Blin, N. A third P-domain peptide gene (TFF3), human intestinal trefoil factor, maps to 21q22.3. Cytogenet. Cell Genet. 72: 299-302, 1996.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

57954 MW

Observed Molecular Weight

12 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Trefoil factor 3

Subcellular Location

Secreted, extracellular space, extracellular matrix.

Protein Name

Alkaline phosphatase, placental type

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.