Anti-XRCC1 Antibody Picoband®

About this product

Boster Bio Anti-XRCC1 Antibody Picoband® catalog # A00571. Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00571

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

XRCC1 (X-RAY REPAIR, COMPLEMENTING DEFECTIVE, IN CHINESE HAMSTER, 1) is a DNA repair protein which complexes with DNA ligase III. The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. The XRCC1 gene is mapped to 19q13.31. The XRCC1 interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. XRCC1 is phosphorylated in vivo and in vitro by CK2, and CK2 phosphorylation of XRCC1 on ser518, thr519, and thr523 largely determines aprataxin binding to XRCC1 through its FHA domain.

Synonyms

DNA repair protein XRCC1; X-ray repair cross-complementing protein 1; XRCC1

Gene Name

XRCC1

Gene ID

7515

UniProt

P18887

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human XRCC1 recombinant protein (Position: E538-A633) .

Clonality

Polyclonal

Applications

ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Field of Research

Cancer, Cancer Metabolism, DNA/RNA, DNA Damage & Repair, Epigenetics and Nuclear Signaling, Metabolic Signaling Pathways, Metabolism, Metabolism Processes, Nucleotide Metabolism, Pathways and Processes, Response To Hypoxia

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.

References & Citations

1. Bhattacharyya, N., Banerjee, S. A novel role of XRCC1 in the functions of a DNA polymerase beta variant. Biochemistry 40: 9005-9013, 2001. 2. Brookman, K. W., Tebbs, R. S., Allen, S. A., Tucker, J. D., Swiger, R. R., Lamerdin, J. E., Carrano, A. V., Thompson, L. H. Isolation and characterization of mouse Xrcc1, a DNA repair gene affecting ligation. Genomics 22: 180-188, 1994.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

69,498 MW

Observed Molecular Weight

90 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

X-ray repair complementing defective repair in Chinese hamster cells 1

Subcellular Location

Nucleus.

Isotype

Rabbit IgG

Contents

243