Anti-Bcl-2/BCL2 Antibody Picoband®

About this product

Anti-Bcl-2/BCL2 Antibody

Specifications

Catalog number

A00040-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t (14;18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.

Synonyms

Apoptosis regulator Bcl-2; BCL2

Gene Name

BCL2

Gene ID

596

UniProt

P10415

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human Bcl-2 recombinant protein (Position: Q118-E165) .

Clonality

Polyclonal

Tissue Specificity

Expressed in a variety of tissues.

Applications

ELISA, Flow Cytometry, IF, ICC, WB

Field of Research

Apoptosis, Apoptotic Markers, Cancer, Cancer Metabolism, Cell Biology, Cell Death, Hypoxia, Intracellular, Invasion/Microenvironment, Metabolism, Metabolism Processes, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Oncoproteins, Oncoproteins/Suppressors, Pathways and Processes, Response To Hypoxia, Signal Transduction, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) . May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785) .

References & Citations

1. Hockenbery, D.; Nunez, G.; Milliman, C.; Schreiber, R. D.; Korsmeyer, S. J. : Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 348: 334-336, 1990. 2. Ngan, B.-Y.; Chen-Levy, Z.; Weiss, L. M.; Warnke, R. A.; Cleary, M. L. : Expression in non-Hodgkin's lymphoma of the BCL-2 protein associated with the t (14;18) chromosomal translocation. New Eng. J. Med. 318: 1638-1644, 1988.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

87335 MW

Observed Molecular Weight

26 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

BCL2, apoptosis regulator

Subcellular Location

Mitochondrion outer membrane.

Protein Name

Signal transducer and activator of transcription 1-alpha/beta

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.