Anti-EEA1 Antibody Picoband®

About this product

Boster Bio Anti-EEA1 Antibody Picoband® catalog # A02296-3. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02296-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The gene EEA1 encodes for the 1400 amino acid protein, Early Endosome Antigen 1. It localizes exclusively to early endosomes and has an important role in endosomal trafficking. EEA1 binds ly to the phospholipid phosphatidylinositol 3-phosphate through its C-terminal FYVE domain and forms ahomodimer through a coiled coil. Furthermore, EEA1 acts as a tethering molecule that couples vesicle docking with SNAREs such as N-ethylmaleimide sensitive fusion protein, bringing the endosomes physically closer and ultimately resulting in the fusion and delivery of endosomal cargo.

Synonyms

Early endosome antigen 1; Endosome-associated protein p162; Zinc finger FYVE domain-containing protein 2; EEA1; ZFYVE2

Gene Name

EEA1

Gene ID

8411

UniProt

Q15075

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human EEA1, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.

Applications

IF, IHC, ICC, WB

Field of Research

Signal Transduction, Subcellular Markers, Tags & Cell Markers, Neuroscience, Protein Trafficking, Organelle Proteins, Organelles, Neurotransmission, Cell Type Marker, Neuron Marker, Intracellular Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

References & Citations

1. Barysch SV, Aggarwal S, Jahn R, Rizzoli SO (Jun 2009) . Sorting in early endosomes reveals connections to docking- and fusion-associated factors. Proceedings of the National Academy of Sciences of the United States of America. 106 (24) : 9697–702. 2. Mishra A, Eathiraj S, Corvera S, Lambright DG (Jun 2010) . Structural basis for Rab GTPase recognition and endosome tethering by the C2H2 zinc finger of Early Endosomal Autoantigen 1 (EEA1) . Proceedings of the National Academy of Sciences of the United States of America. 107 (24) : 10866–71.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

162466 MW

Observed Molecular Weight

170 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Early endosome antigen 1

Subcellular Location

Cytoplasm. Early endosome membrane; Peripheral membrane protein.

Protein Name

Arachidonate 12-lipoxygenase, 12S-type

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.