Anti-Integrin alpha 5/ITGA5 Antibody Picoband®

About this product

Boster Bio Anti-Integrin alpha 5/ITGA5 Antibody Picoband® catalog # A01911. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01911

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Integrin alpha-5, also known as FNRA or VLA5A, is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

Synonyms

Integrin alpha-5; CD49 antigen-like family member E; Fibronectin receptor subunit alpha; Integrin alpha-F; VLA-5; CD49e; Integrin alpha-5 heavy chain; Integrin alpha-5 light chain; ITGA5; FNRA

Gene Name

ITGA5

Gene ID

3678

UniProt

P08648

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human Integrin alpha 5 recombinant protein (Position: F42-A263) .

Clonality

Polyclonal

Tissue Specificity

Highest expression in the prostate, small intestine, colon, kidney and liver.

Applications

ELISA, IHC, WB

Field of Research

Cell Adhesion, Cytoskeleton/ECM, Integrins, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877) . ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881) .

References & Citations

1. Sosnoski DM, Emanuel BS, Hawkins AL, van Tuinen P, Ledbetter DH, Nussbaum RL, Kaos FT, Schwartz E, Phillips D, Bennett JS, et al. (Aug 1988) . 2. Entrez Gene: ITGA5 integrin, alpha 5 (fibronectin receptor, alpha polypeptide) .

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

40895 MW

Observed Molecular Weight

150 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Integrin, alpha 5 (fibronectin receptor, alpha polypeptide)

Subcellular Location

Membrane; Single-pass type I membrane protein. Cell junction, focal adhesion.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.