Anti-MAX Antibody Picoband®

About this product

Boster Bio Anti-MAX Antibody Picoband® catalog # A03239. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03239

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

MAX (Max protein), also called Myc-associated factor x, is the most conserved dimerization component of the MYC-MAX-MXD1 network of basic helix-loop-helix leucine zipper (bHLHZ) transcription factors that regulate cell proliferation, differentiation, and apoptosis. The conservation of the MAX sequence is particularly high in the bHLHZ domain, which is involved in protein-protein interactions and DNA binding. The MAX gene is located on chromosome 14q23 by fluorescence in situ chromosomal hybridization. Both quasisymmetric heterodimers resemble the symmetric MAX homodimer, albeit with marked structural differences in the coiled-coil leucine zipper regions that explain preferential homo- and heteromeric dimerization of these 3 evolutionarily related DNA-binding proteins. MAX acts as a classic tumor suppressor gene. Normal lymphocytes from patients showed absence of methylation of the MAX promoter and biallelic expression of MAX, which ruled out an imprinting-mediated effect on MAX expression. The ability of these cells to divide, differentiate, and apoptose in the absence of Max demonstrated for the first time that these processes can occur via Max- and possibly Myc-independent mechanisms.

Synonyms

Protein max; Class D basic helix-loop-helix protein 4; bHLHd4; Myc-associated factor X; MAX; BHLHD4

Gene Name

MAX

Gene ID

4149

UniProt

P61244

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human MAX recombinant protein (Position: A30-R106) .

Clonality

Polyclonal

Tissue Specificity

High levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle.

Applications

ELISA, IF, ICC, WB, Flow Cytometry

Field of Research

Cancer Susceptibility,2339, Domain Families, Epigenetics and Nuclear Signaling, Hlh/Leucine Zipper, Transcription, Tumor Suppressors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcription regulator. Forms a sequence-specific DNA- binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity.

References & Citations

1. Blackwood, E., Eisenman, R. N. Max: a helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex with Myc.Science 251: 1211-1217, 1991. 2. Gilladoga, A. D., Edelhoff, S., Blackwood, E. M., Eisenman, R. N., Disteche, C. M. Mapping of MAX to human chromosome 14 and mouse chromosome 12 by in situ hybridization. Oncogene 7: 1249-1251, 1992. 3. Hopewell, R., Ziff, E. B. The nerve growth factor-responsive PC12 cell line does not express the Myc dimerization partner Max. Molec. Cell Biol. 15: 3470-3478, 1995.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

19-21 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

MYC associated factor X

Subcellular Location

Nucleus. Cell projection, dendrite.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.