Anti-Ogg1 Antibody Picoband®

About this product

Boster Bio Anti-Ogg1 Antibody Picoband® catalog # A00768-1. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00768-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

8-Oxoguanine glycosylase also known as OGG1 is a DNA glycosylase enzyme that, in humans, is encoded by theOGG1 gene. This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined.

Synonyms

N-glycosylase/DNA lyase; 8-oxoguanine DNA glycosylase; DNA- (apurinic or apyrimidinic site) lyase; AP lyase; OGG1; MMH; MUTM; OGH1

Gene Name

OGG1

Gene ID

4968

UniProt

O15527

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Ogg1, which shares 89.7% and 87.2% amino acid (aa) sequence identity with mouse and rat Ogg1, respectively.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous.

Applications

IP, IF, IHC, ICC, WB

Field of Research

Cancer, DNA/RNA, DNA Damage & Repair, Epigenetics and Nuclear Signaling, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N- methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta- lyase activity that nicks DNA 3' to the lesion.

References & Citations

1. Entrez Gene: OGG1 8-oxoguanine DNA glycosylase. 2. Nishioka K, Ohtsubo T, Oda H, Fujiwara T, Kang D, Sugimachi K, Nakabeppu Y (1999) . Expression and differential intracellular localization of two major forms of human 8-oxoguanine DNA glycosylase encoded by alternatively spliced OGG1 mRNAs. Mol. Biol. Cell. 10 (5) : 1637–52.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

39 kDa

Observed Molecular Weight

39 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

8-oxoguanine DNA glycosylase

Subcellular Location

Nucleus, nucleoplasm.

Protein Name

Muellerian-inhibiting factor

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.