Anti-PIAS3 Antibody Picoband®

About this product

Boster Bio Anti-PIAS3 Antibody Picoband® catalog # A02807-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02807-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

E3 SUMO-protein ligase PIAS3 is an enzyme that in humans is encoded by the PIAS3 gene. This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription) ] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier) -E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It ly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined.

Synonyms

E3 SUMO-protein ligase PIAS3; Protein inhibitor of activated STAT protein 3; PIAS3

Gene Name

PIAS3

Gene ID

10401

UniProt

Q9Y6X2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human PIAS3, which shares 97.6% amino acid (aa) sequence identity with both mouse and rat PIAS3.

Clonality

Polyclonal

Tissue Specificity

Widely expressed.

Applications

WB

Field of Research

Cell Biology, Co-Activators/Co-Repressors, Epigenetics and Nuclear Signaling, Nuclear Receptors, Nuclear Signaling Pathways, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Transcription, Transcription Factors, Ubiquitin & Ubiquitin Like Modifiers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184) . Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032) . Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376) .

References & Citations

1. Entrez Gene: PIAS3 protein inhibitor of activated STAT, 3. 2. Ueki N, Seki N, Yano K, Saito T, Masuho Y, Muramatsu M (June 1999) . Isolation and chromosomal assignment of a human gene encoding protein inhibitor of activated STAT3 (PIAS3) . Journal of Human Genetics. 44 (3) : 193–6.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

96950 MW

Observed Molecular Weight

68 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Protein inhibitor of activated STAT 3

Subcellular Location

Cytoplasm.

Protein Name

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

Isotype

Rabbit IgG

Contents

243