Anti-PHO1/APOBEC3A Antibody Picoband®

About this product

Boster Bio Anti-PHO1/APOBEC3A Antibody Picoband® catalog # A01183-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01183-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A, also known as APOBEC3A, is a gene of the APOBEC3 family found in humans, non-human primates, and some other mammals.This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22.The protein plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

DNA dC->dU-editing enzyme APOBEC-3A; A3A; Phorbolin-1; APOBEC3A

Gene Name

APOBEC3A

Gene ID

200315

UniProt

P31941

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human PHO1 recombinant protein (Position: M1-L63) .

Clonality

Polyclonal

Tissue Specificity

Expressed in peripheral leukocytes with higher expression in CD14-positive phagocytic cells. Highly expressed in keratinocytes and in periphery blood monocytes. Also detected in non-lymphoid tissues including lung and adipose tissues. Found at high levels in colorectal adenocarcinoma, Burkitt's lymphoma and chronic myelogenous leukemia.

Applications

ELISA, WB

Field of Research

Cell Biology, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

DNA deaminase (cytidine deaminase) with restriction activity against viruses, foreign DNA and mobility of retrotransposons. Exhibits antiviral activity against adeno- associated virus (AAV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons. Selectively targets single-stranded DNA and can deaminate both methylcytosine and cytosine in foreign DNA. Can induce somatic hypermutation in the nuclear and mitochondrial DNA. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.

References & Citations

1. Entrez Gene: APOBEC3A apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A. 2. Iñigo Narvaiza; Daniel C. Linfesty; Benjamin N. Greener; Yoshiyuki Hakata; David J. Pintel; Eric Logue; Nathaniel R. Landau; Matthew D. Weitzman (2009) . Jung, Jae U., ed. Deaminase-Independent Inhibition of Parvoviruses by the APOBEC3A Cytidine Deaminase. PLoS Pathogens. 5 (5) : e1000439. 3. Mark D. Stenglein; Michael B. Burns; Michael B. Burns; Joy Lengyel; Reuben S. Harris (2010) . APOBEC3 proteins mediate the clearance of foreign DNA from human cells. Nature Structural & Molecular Biology. 17 (2) : 222–229.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

28 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Apolipoprotein B mRNA editing enzyme catalytic subunit 3A

Subcellular Location

Nucleus. Cytoplasm.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.