Anti-DGCR8 Antibody Picoband®

About this product

Boster Bio Anti-DGCR8 Antibody Picoband® catalog # A00475-1. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00475-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The DGCR8 microprocessor complex subunit (DiGeorge syndrome chromosomal [or critical] region 8) is a protein that in humans is encoded by the DGCR8 gene. This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants.

Synonyms

Microprocessor complex subunit DGCR8; DiGeorge syndrome critical region 8; DGCR8; C22orf12, DGCRK6; LP4941

Gene Name

DGCR8

Gene ID

54487

UniProt

Q8WYQ5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human DGCR8 recombinant protein (Position: K561-Q762) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed.

Applications

ELISA, IF, IHC, ICC, WB

Field of Research

DNA/RNA, Epigenetics and Nuclear Signaling, Nuclear Receptors, Nuclear Signaling Pathways, RNA Processing

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and s DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718) . The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994) . Specifically recognizes and binds N6-methyladenosine (m6A) -containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998) . Involved in the silencing of embryonic stem cell self-renewal (By similarity) .

References & Citations

1. Han, J., Lee, Y., Yeom, K.-H., Kim, Y.-K., Jin, H., Kim, V. N. The Drosha-DGCR8 complex in primary microRNA processing. Genes Dev. 18: 3016-3027, 2004. 2. Shiohama, A., Sasaki, T., Noda, S., Minoshima, S., Shimizu, N. Molecular cloning and expression analysis of a novel gene DGCR8 located in the DiGeorge syndrome chromosomal region. Biochem. Biophys. Res. Commun. 304: 184-190, 2003.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

33477 MW

Observed Molecular Weight

100 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

DGCR8, microprocessor complex subunit

Subcellular Location

Nucleus.

Protein Name

Tumor necrosis factor ligand superfamily member 10

Isotype

Rabbit IgG

Contents

243