Anti-IL4 Antibody Picoband®

About this product

Boster Bio Anti-IL4 Antibody Picoband® catalog # A00230. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00230

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-4 (IL-4), also known as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.1 The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-ed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.2. The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.

Synonyms

Interleukin-4; IL-4; B-cell stimulatory factor 1; BSF-1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra; IL4

Gene Name

IL4

Gene ID

3565

UniProt

P05112

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human IL4 recombinant protein (Position: H25-S153) .

Clonality

Polyclonal

Tissue Specificity

Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.

Applications

ELISA, WB

Field of Research

Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Participates in at least several B-cell activation processes as well as of other cell types (PubMed:3016727) . It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages (By similarity) .

References & Citations

1. Arai, N.; Nomura, D.; Villaret, D.; DeWaal Malefijt, R.; Seiki, M.; Yoshida, M.; Minoshima, S.; Fukuyama, R.; Maekawa, M.; Kudoh, J.; Shimizu, N.; Yokota, K.; Abe, E.; Yokota, T.; Takebe, Y.; Arai, K. Complete nucleotide sequence of the chromosomal gene for human IL-4 and its expression. J. Immun. 142: 274-282, 1989. 2. Prokopchuk, O.; Liu, Y.; Henne-Bruns, D.; Kornmann, M. Interleukin-4 enhances proliferation of human pancreatic cancer cells: evidence for autocrine and paracrine actions. Br J Cancer.2005 Mar 14;92 (5) :921-8.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

36053 MW

Observed Molecular Weight

20 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Interleukin 4

Subcellular Location

Secreted.

Protein Name

Glyceraldehyde-3-phosphate dehydrogenase

Isotype

Rabbit IgG

Contents

243