Anti-L1CAM Antibody

About this product

Boster Bio Anti-L1CAM Antibody Picoband® catalog # A00729-1. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A00729-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

L1, also known as L1CAM, is a transmembrane protein member of the L1 protein family, encoded by the L1CAM gene. The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus) . Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons.

Synonyms

Neural cell adhesion molecule L1; N-CAM-L1; NCAM-L1; CD171; L1CAM; CAML1; MIC5

Gene Name

L1CAM

Gene ID

3897

UniProt

P32004

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human L1CAM, which shares 88.2% and 82.4% amino acid (aa) sequence identity with mouse and rat L1CAM, respectively.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous. Highest expression seen in the heart and skeletal muscle.

Applications

IHC

Field of Research

Axonal Guidance Proteins, Cell Adhesion, Cell Adhesion Molecules, Cytoskeleton/ECM, Growth and Development, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.

References & Citations

1. Basel-Vanagaite, L., Straussberg, R., Friez, M. J., Inbar, D., Korenreich, L., Shohat, M., Schwartz, C. E. Expanding the phenotypic spectrum of L1CAM-associated disease. Clin. Genet. 69: 414-419, 2006. 2. Du, Y.-Z., Dickerson, C., Aylsworth, A. S., Schwartz, C. E. A silent mutation, C924T (G308G), in the L1CAM gene results in X linked hydrocephalus (HSAS) . J. Med. Genet. 35: 456-462, 1998.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

52 kDa, 60 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

L1 cell adhesion molecule

Subcellular Location

Cell membrane.

Isotype

Rabbit IgG

Contents

243