Anti-MDC1 Antibody

About this product

Boster Bio Anti-MDC1 Antibody Picoband® catalog # A01252-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC applications. This antibody reacts with Human.

Specifications

Catalog number

A01252-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mediator of DNA damage checkpoint protein 1 is a 2080 amino acid long protein that in humans is encoded by the MDC1 gene. The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.

Synonyms

Mediator of DNA damage checkpoint protein 1; Nuclear factor with BRCT domains 1; MDC1; KIAA0170; NFBD1

Gene Name

MDC1

Gene ID

9656

UniProt

Q14676

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human MDC1 recombinant protein (Position: A534-Q626) .

Clonality

Polyclonal

Tissue Specificity

Highly expressed in testis.

Applications

ELISA, Flow Cytometry, IF, IHC, ICC

Field of Research

DNA/RNA, DNA Damage & Repair, DNA Damage Response, Epigenetics and Nuclear Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.

References & Citations

1. Dimitrova, N., de Lange, T. MDC1 accelerates nonhomologous end-joining of dysfunctional telomeres. Genes Dev. 20: 3238-3243, 2006. 2. Goldberg, M., Stucki, M., Falck, J., D'Amours, D., Rahman, D., Pappin, D., Bartek, J., Jackson, S. P. MDC1 is required for the intra-S-phase DNA damage checkpoint. Nature 421: 952-956, 2003.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

50 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Mediator of DNA damage checkpoint 1

Subcellular Location

Nucleus.

Isotype

Rabbit IgG

Contents

243